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In Vivo Quantification of Histone Acetylation Turnover and Acetyl-CoA Sources Using ²H₂O Metabolic Labeling and High-Resolution Mass Spectrometry

2026-06-29

Abstract excerpt

Dysregulated histone acetylation links cellular metabolism to gene expression, but measuring its in vivo turnover remains technically challenging. Here, we introduce a 2 H2O -based metabolic labeling method coupled with high-resolution Orbitrap mass spectrometry to quantify in vivo histone acetylation dynamics. The approach leverages differing deuterium incorporation rates between fast-labeling acetyl groups a...

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Literature Corpus work: e359b0d8-b204-5c57-9f98-38b94875b1e1
DOI: 10.64898/2026.06.26.734905

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<i>In Vivo</i> Quantification of Histone Acetylation Turnover and Acetyl-CoA Sources Using ²H₂O Metabolic Labeling and High-Resolution Mass Spectrometry

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