Article

Direct conversion of somatic cells into ‘insulin-producing-cells’ by user-defined multiplex-epigenetic-engineering vector (MEEV-β)

2022-08-13

Abstract excerpt

We demonstrate here a single-step and user-friendly approach to generate insulin producing cells by gRNA driven specific-activation of PDX1, NKX6.1, MAFA, Insulin and Glut2 genes in somatic cells via multiplex-epigenetic-engineering-vector (MEEV-β) containing dCas9.P300 core developed by us. Sorted Glut2 + cells could secrete insulin in response to glucose challenge and showed expression of β-cell specific trans...

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Literature Corpus work: e0041211-a17a-58be-a2ce-d801d7a47b40
DOI: 10.1101/2022.08.12.503816

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