Article

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

2023-09-17

Abstract excerpt

<h4>Summary</h4> Successful genome editing in primary human islets could reveal features of the genetic regulatory landscape underlying β cell function and diabetes risk. Here, we describe a CRISPR-based strategy to interrogate functions of predicted regulatory DNA elements using electroporation of a complex of Cas9 ribonucleoprotein (Cas9 RNP) and guide RNAs into primary human islet cells. We successfully target...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 41751eda-8bab-575c-bc3c-c0462997692f
DOI: 10.1101/2023.09.16.558090

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoproteinDOI 10.1101/2023.09.16.558090Read

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoproteinSemantic proximity Read

CRISPR-based genome editing in primary human pancreatic islet cellsSemantic proximity Read

Activation of pancreatic β-cell genes by multiplex epigenetic CRISPR-editingSemantic proximity Read

Functional Genomics in Pancreatic β Cells: Recent Advances in Gene Deletion and Genome Editing Technologies for Diabetes ResearchSemantic proximity Read

CRISPR/Cas technologies in pancreatic cancer research and therapeutics: recent advances and future outlookSemantic proximity Read

Genome editing of human pancreatic beta cell models: problems, possibilities and outlookSemantic proximity Read

A CRISPR/Cas9 genome editing pipeline in the EndoC-βH1 cell line to study genes implicated in beta cell functionSemantic proximity Read

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development.Semantic proximity Read

CRISPR Screening Uncovers a Long-Range Enhancer for <i>ONECUT1</i> in Pancreatic Differentiation and Links a Diabetes Risk VariantSemantic proximity Read

CRISPR-Cas9 Therapeutics in Diabetes Mellitus: From Molecular Mechanisms and Pharmacology to Clinical Development and Translational BarriersSemantic proximity Read

Genome-edited human stem cell-derived beta cells: a powerful tool for drilling down on type 2 diabetes GWAS biologySemantic proximity Read

IVT generation of guideRNAs for Cas9-enrichment Nanopore SequencingSemantic proximity Read