Article

Hidden assumptions in nascent RNA sequencing pipelines define reproducibility states

2026-07-17

Abstract excerpt

Reproducibility of sequencing analyses is often assumed when identical data are processed with established pipelines, yet outcomes can depend on library assumptions that are not explicit to users. Here we examined commonly used pipelines for nascent RNA sequencing. Across public human PRO-seq datasets, identical inputs generated structured divergence in transcriptional profiles. Diagnostic processing combinations...

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Literature Corpus work: de3e5dce-cd0f-504b-8965-c8e2b67f01e6
DOI: 10.64898/2026.07.13.738089

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