Article
Impact of library preparation on downstream analysis and interpretation of RNA-Seq data: comparison between Illumina PolyA and NuGEN Ovation protocol.
PloS one - 1 Jan 2013
Sun Zhifu, Asmann Yan W, Nair Asha, Zhang Yuji, Wang Liguo, Kalari Krishna R, Bhagwate Aditya V, Baker Tiffany R, Carr Jennifer M, Kocher Jean-Pierre A, Perez Edith A, Thompson E Aubrey
Abstract excerpt
OBJECTIVES: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses of the broad applications of RNA sequencing. METHODS AND MATERIALS: Eight human mammary epithelial cell (HMEC) lines...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.