“Misuse” of RNA‐seq data in microbiome studies: A cautionary tale of poly(A)

Abstract excerpt

The polyadenylation (poly(A)) in messenger RNA (mRNA) represents a well-known and fundamental distinction between the host and the majority of microbiota. The increasing use of poly(A)-enriched RNA sequencing (RNA-seq) datasets to map out microbial abundances raises concerns that this approach may introduce bias, potentially skewing the results and leading to inaccurate conclusions. Here, I aim to raise awareness...

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