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Article

Extensive length and homology dependent chimerism in pool-packaged AAV libraries

2025-01-15

Abstract excerpt

Adeno-associated viruses (AAVs) have emerged as the foremost gene therapy delivery vehicles due to their versatility, durability, and safety profile. Here we demonstrate extensive chimerism, manifesting as pervasive barcode swapping, among complex AAV libraries that are packaged as a pool. The observed chimerism is length- and homology-dependent but capsid-independent, in some cases affecting the majority of packa...

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Identifiers and source

Literature Corpus work
ddf1989c-a1ed-521f-a71c-c129d25d8326
DOI
10.1101/2025.01.14.632594
Open publication

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Extensive length and homology dependent chimerism in pool-packaged AAV librariesDOI 10.1101/2025.01.14.632594
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