Article

Reproducibility and Accuracy of Nanopore-Based Methylome Profiling of <i>Streptococcus dysgalactiae</i> subspecies <i>equisimilis</i> Strains from Cancer Patients

2025-06-06

Abstract excerpt

<h4>Background</h4> DNA methylation influences bacterial gene regulation, virulence, and restriction-modification (RM) systems. Advances by Oxford Nanopore Technologies (ONT) now enable direct methylome profiling from nanopore sequencing using the Dorado basecaller. However, the comparative performance of ONT-only versus hybrid-assembly reference-based methylation calling, particularly regarding genomic DNA quali...

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Literature Corpus work: dd376c09-f832-5f6c-adfa-e5beb30eff1f
DOI: 10.1101/2025.06.04.657942

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