Article

Differences between <i>in vivo</i> and <i>ex vivo</i> models drive divergent perturbation outcomes

2025-09-07

Abstract excerpt

Ex vivo experimental models are extensively used as reductionist models, yet the impact of model choice on molecular states and perturbation responses has not been systematically evaluated. Here, we compared the outcomes of genetic perturbations in hematopoietic stem cells between in vivo and ex vivo model systems. This revealed strong baseline differences characterized by a reduced interferon response and eleva...

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Literature Corpus work: dc665e99-c4c9-58f8-b665-b4fffa98a3fe
DOI: 10.1101/2025.09.02.673692

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