Article

Methods to study toxic transgenes in <i>C. elegans</i> : an analysis of protease-dead separase in the <i>C. elegans</i> embryo

2014-02-06

Abstract excerpt

<h4>ABSTRACT</h4> We investigated whether the protease activity of separase, which is required for chromosome segregation, is also required for its other roles during anaphase in C. elegans given that non-proteolytic functions of separase have been identified in other organisms. We find that expression of protease-dead separase is dominant-negative in C. elegans embryos. The C. elegans embryo is an ideal syste...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d96ab54b-3d52-5418-85c0-f4b140e59ff2
DOI: 10.1101/002444

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Methods to study toxic transgenes in <i>C. elegans</i> : an analysis of protease-dead separase in the <i>C. elegans</i> embryoDOI 10.1101/002444Read

Genetic Identification of Separase Regulators in Caenorhabditis elegans.Semantic proximity Read

Employing the one-cell C. elegans embryo to study cell division processes.Semantic proximity Read

Separase Cleaves the N-Tail of the CENP-A Related Protein CPAR-1 at the Meiosis I Metaphase-Anaphase Transition in C. elegans.Semantic proximity Read

Caspase-Dependent Cell Death in<i>Drosophila</i>Semantic proximity Read

Conservation of the separase regulatory domain.Semantic proximity Read

The <i>C. elegans</i> proteasome subunit RPN-12 is required for hermaphrodite germline sex determination and oocyte qualitySemantic proximity Read

Combined flow cytometry and high throughput image analysis for the study of essential genes in <i>Caenorhabditis elegans</i>Semantic proximity Read

Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in <i>C. elegans</i>Semantic proximity Read

Separase is required for chromosome segregation during meiosis I in Caenorhabditis elegans.Semantic proximity Read

A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans.Semantic proximity Read

Rapid generation of <i>C. elegans</i> single copy transgenes combining RMCE and drug selectionSemantic proximity Read

Dissection of cell division processes in the one cell stage Caenorhabditis elegans embryo by mutational analysis.Semantic proximity Read