Article

Monolayer platform to generate and purify human primordial germ cells in vitro provides new insights into germline specification

2021-02-05

Abstract excerpt

<title>Abstract</title> <p>Generating primordial germ cells (PGCs) from human pluripotent stem cells (hPSCs) advances studies of human reproduction and development of infertility treatments, but currently entails complex 3D aggregates. Here we develop a simplified, monolayer method to differentiate hPSCs into PGCs within 3.5 days. We used our simplified differentiation platform and single-cell RNA-sequencing to u...

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Literature Corpus work: d8387491-f9d6-54e1-8d55-c0b797ddb912
DOI: 10.21203/rs.3.rs-113078/v1

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