Article

Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

2022-09-30

Abstract excerpt

<h4>SUMMARY</h4> Current human primordial germ cell like cells (hPGCLCs) differentiation methods from human pluripotent stem cells (hPSCs) are inefficient, and it is challenging to generate sufficient hPGCLCs to optimize in vitro gametogenesis. We present a new differentiation method that uses diluted basement membrane extract (BME) and low BMP4 concentration to efficiently induce hPGCLC differentiation in scalab...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 73112c7b-b481-5417-9672-bd25c93395b3
DOI: 10.1101/2022.09.28.509976

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlayDOI 10.1101/2022.09.28.509976Read

Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review of in vitro methodsSemantic proximity Read

Monolayer platform to generate and purify human primordial germ cells in vitro provides new insights into germline specificationSemantic proximity Read

Reconstitution of human fetal ovaries reveals niche requirements for primordial germ cell-like cell progressionSemantic proximity Read

TFAP2A+ embryonic progenitor cells undergo fate diversification to give rise to human amnion, germline, and mesodermSemantic proximity Read

Specification of human germ cell fate with enhanced progression capability supported by hindgut organoidsSemantic proximity Read

Efficient differentiation of human primordial germ cells through geometric control reveals a key role for Nodal signalingSemantic proximity Read

The emergence of human primordial germ cell–like cells in stem cell–derived gastruloidsSemantic proximity Read

Efficient Human Germ Cell Specification from Stem Cells via Combinatorial Expression of Transcription FactorsSemantic proximity Read

High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humansSemantic proximity Read

Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesisSemantic proximity Read

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro†Semantic proximity Read

Research Advances in Gametogenesis and Embryogenesis Using Pluripotent Stem CellsSemantic proximity Read