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Large-scale bidirectional arrayed genetic screens identify <i>OXR1</i> and <i>EMC4</i> as modifiers of α-synuclein aggregation

2025-06-11

Abstract excerpt

In Parkinson’s disease and other synucleinopathies, α-synuclein (α-Syn) misfolds and forms Ser 129 -phosphorylated aggregates (pSyn 129 ). The factors controlling this process are largely unknown. Here, we used arrayed CRISPR-mediated gene activation and ablation to discover new pSyn 129 modulators. Using quadruple-guide RNAs (qgRNAs) and Cas9, or an inactive Cas9 version fused to a synthetic transactivator, we...

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Literature Corpus work
d36fe964-e14d-5eb2-99ce-50a054f256e4
DOI
10.1101/2025.06.10.658866
Open publication

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Large-scale bidirectional arrayed genetic screens identify <i>OXR1</i> and <i>EMC4</i> as modifiers of α-synuclein aggregationDOI 10.1101/2025.06.10.658866
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