Protective antigen-mediated delivery of an anti-CRISPR protein for precision genome editing

Abstract excerpt

Precise control over the dosage of Cas9-based technologies is essential because off-target effects, mosaicism, chromosomal aberrations, immunogenicity, and genotoxicity can arise with prolonged Cas9 activity. Type II anti-CRISPR proteins (Acrs) inhibit and control Cas9 but are generally impermeable to the cell membrane due to their size (6–54 kDa) and anionic charge. Moreover, existing Acr delivery methods are lon...

Topics

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• Bacteria
• CRISPR and Genetic Engineering
• CRISPR-Associated Proteins
• CRISPR-Cas Systems
• Deoxyribonuclease I
• Mosquito-borne diseases and control
• PDZ Domains
• SARS-CoV-2 and COVID-19 Research

Identifiers and source

Literature Corpus work

98a586d3-0548-584a-8cb8-917506b88ed7

DOI

10.1101/2025.02.23.639680