Nuclear Localization Signals Enable the Cellular Delivery of an Anti-CRISPR Protein to Control Genome Editing

Abstract excerpt

<h4>ABSTRACT</h4> Precise regulation of Cas9 activity is essential to minimize off-target effects, mosaicism, chromosomal alterations, immunogenicity, and genotoxicity in genome editing. Although type II anti-CRISPR proteins (Acrs) can inhibit and regulate Cas9, their size and anionic charge generally prevent them from crossing the cell membrane. Existing Acr delivery methods employing vectors or electroporation...

Topics

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• CAR-T cell therapy research
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• DNA
• Mutation
• RNA Editing
• RNA, Guide, CRISPR-Cas Systems
• Virus-based gene therapy research

Identifiers and source

Literature Corpus work

532f86ec-9a4f-5992-a00d-62184ebce421

DOI

10.1101/2025.10.28.685205