Article

Multiplexed single-molecule analysis of human telomerase synthesizing DNA

2021-06-25

Abstract excerpt

Genomic stability in proliferating cells critically depends on telomere maintenance by telomerase reverse transcriptase. Here we developed a real-time single-molecule RNA sequencing approach that visualizes telomerase catalysis and structural dynamics at single-nucleotide resolution using FRET and zero-mode waveguides. The method permits direct detection of dynamic steps and structural states throughout the telome...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: cf515d73-844c-5eaf-8cd9-d39b743b98ff
DOI: 10.1101/2021.06.25.449954

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Multiplexed single-molecule analysis of human telomerase synthesizing DNADOI 10.1101/2021.06.25.449954Read

Single-Molecule Studies of Telomeres and TelomeraseSemantic proximity Read

Single-molecule real-time detection of telomerase extension activitySemantic proximity Read

A guide to nucleic acid detection by single-molecule kinetic fingerprinting.Semantic proximity Read

Single-molecule FRET reveals the folding dynamics of the human telomerase RNA pseudoknot domain.Semantic proximity Read

Telomerase RNA structural heterogeneity in living human cells detected by DMS-MaPseqSemantic proximity Read

Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolutionSemantic proximity Read

Rapid Kinetic Fingerprinting of Single Nucleic Acid Molecules by a FRET-based Dynamic NanosensorSemantic proximity Read

Single-Molecule Imaging of Telomerase RNA Reveals a Recruitment-Retention Model for Telomere Elongation.Semantic proximity Read

Ratiometric Fluorescent Biosensor for Visual Discrimination of Cancer Cells with Different Telomerase Expression LevelsSemantic proximity Read

A self-regulating template in human telomerase.Semantic proximity Read

Nanophotonic waveguide-based sensing of circulating cell-free mitochondrial DNA: implications for personalized medicine.Semantic proximity Read

A cytometric assay for ultrasensitive and robust detection of human telomerase RNA based on toehold strand displacementSemantic proximity Read