Article
A guide to nucleic acid detection by single-molecule kinetic fingerprinting.
Methods (San Diego, Calif.) - 15 Jan 2019
Johnson-Buck Alexander, Li Jieming, Tewari Muneesh, Walter Nils G
Abstract excerpt
Conventional methods for detecting small quantities of nucleic acids require amplification by the polymerase chain reaction (PCR), which necessitates prior purification and introduces copying errors. While amplification-free methods do not have these shortcomings, they are generally orders of magnitude less sensitive and specific than PCR-based methods. In this review, we provide a practical guide to a novel...
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