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Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution

26 May 2014

Abstract excerpt

The telomere repeat amplification protocol (TRAP) for the human reverse transcriptase, telomerase, is a PCR-based assay developed two decades ago and is still used for routine determination of telomerase activity. The TRAP assay can only reproducibly detect ∼ 2-fold differences and is only quantitative when compared to internal standards and reference cell lines. The method generally involves laborious...

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PMID: 24861623
DOI: 10.1093/nar/gku439

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