Article

Determining Allele-Specific Protein Expression (ASPE) Using a QconCAT-Based Proteomics Method: a Novel Approach to Identify <i>Cis</i> -acting Genetic Variants

2018-02-13

Abstract excerpt

Measuring allele-specific expression (ASE) is a powerful approach for identifying cis- regulatory genetic variants. Here we developed a novel targeted proteomics method for quantification of allele-specific protein expression (ASPE) based on scheduled high resolution multiple reaction monitoring (sMRM-HR) with a heavy stable isotope-labeled quantitative concatamer (QconCAT) internal protein standard. This strateg...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: ca8fe80a-03f8-5845-a475-3154e7a54db7
DOI: 10.1101/264614

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Determining Allele-Specific Protein Expression (ASPE) Using a QconCAT-Based Proteomics Method: a Novel Approach to Identify <i>Cis</i> -acting Genetic VariantsDOI 10.1101/264614Read

Determining Allele-Specific Protein Expression (ASPE) Using a Novel Quantitative Concatamer Based Proteomics Method.Semantic proximity Read

Differential allelic expression of c.1568C > A at UGT2B15 is due to variation in a novel cis-regulatory element in the 3'UTR.Semantic proximity Read

Identification of regulatory variants of carboxylesterase 1 (CES1): A proof-of-concept study for the application of the Allele-Specific Protein Expression (ASPE) assay in identifying cis-acting regulatory genetic polymorphisms.Semantic proximity Read

Allelic imbalance (AI) identifies novel tissue-specific cis-regulatory variation for human UGT2B15.Semantic proximity Read

Quantitative analysis of single amino acid variant peptides associated with pancreatic cancer in serum by an isobaric labeling quantitative method.Semantic proximity Read

Quantitative detection of single amino acid polymorphisms by targeted proteomics.Semantic proximity Read

A compensatory effect upon splicing results in normal function of the CYP2A6*14 allele.Semantic proximity Read

SNP discovery, expression and cis-regulatory variation in the UGT2B genes.Semantic proximity Read

Searching for polymorphisms that affect gene expression and mRNA processing: example ABCB1 (MDR1).Semantic proximity Read

Genetic diversity at the UGT1 locus is amplified by a novel 3' alternative splicing mechanism leading to nine additional UGT1A proteins that act as regulators of glucuronidation activity.Semantic proximity Read

A single nucleotide polymorphism based approach for the identification and characterization of gene expression modulation using MassARRAY.Semantic proximity Read

Quantitative measurement of allele-specific protein expression in a diploid yeast hybrid by LC-MS.Semantic proximity Read