High-Throughput Plasmid Content Analysis of <i>Borrelia burgdorferi</i> B31 by Using Luminex Multiplex Technology

Abstract excerpt

Borrelia burgdorferi, the causative agent of Lyme disease in North America, is an invasive pathogen that causes persistent multiorgan manifestations in humans and other mammals. Genetic studies of this bacterium are complicated by the presence of multiple plasmid replicons, many of which are readily lost during in vitro culture. The analysis of B. burgdorferi plasmid content by plasmid-specific PCR and agarose...

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