Article

Improved Protein Semi-Synthesis Enables Biophysical Studies of Thioamide Destabilization of β-Sheet Interactions

2026-07-31

Abstract excerpt

Thioamides are natural post-translational modifications of the peptide backbone and can be introduced synthetically to probe protein folding or functionalize peptides for translational applications. In this work, we demonstrate that thioamide-containing peptides with C-terminal thioesters can be efficiently generated using Knorr pyrazole activation and used in subsequent native chemical ligation reactions to gener...

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Literature Corpus work: c5e2f7e5-be98-5262-9856-f1017185a41c
DOI: 10.64898/2026.07.30.741859

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