Article

A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth sgRNA

2024-09-25

Abstract excerpt

<h4>ABSTRACT</h4> Coronaviruses have large, positive-sense single-stranded RNA genomes that challenge conventional strategies for mutagenesis. Here, we report the development of a new reverse genetics system for the endemic human coronavirus (HCoV) OC43 that utilizes transformation-associated recombination (TAR) to assemble complete viral genomes from dsDNA genome fragments via homologous recombination in Saccha...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c504611e-dfbb-51a0-9a78-3be95413fe88
DOI: 10.1101/2024.09.23.614401

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth sgRNADOI 10.1101/2024.09.23.614401Read

A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth subgenomic RNASemantic proximity Read

Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic PlatformsSemantic proximity Read

Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated RecombinationSemantic proximity Read

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform.Semantic proximity Read

In-yeast reconstruction of the African swine fever virus genome isolated from clinical samplesSemantic proximity Read

Rapid reconstruction of infectious bronchitis virus expressing fluorescent protein from its nsp2 gene based on transformation-associated recombination platformSemantic proximity Read

Recovery of a Neurovirulent Human Coronavirus OC43 from an Infectious cDNA CloneSemantic proximity Read

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platformSemantic proximity Read

One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing TechnologySemantic proximity Read

A versatile, simple, and rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications.Semantic proximity Read

A nano-luciferase expressing human coronavirus OC43 for countermeasure developmentSemantic proximity Read

The ns12.9 Accessory Protein of Human Coronavirus OC43 Is a Viroporin Involved in Virion Morphogenesis and PathogenesisSemantic proximity Read