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In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples

1 Sept 2021

Abstract excerpt

This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. In addition, the precise deletion of a non-essential gene and its replacement by a synthetic reporter gene cassette...

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PMID: 34527959
DOI: 10.1016/j.xpro.2021.100803

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