Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform.

doi:10.1038/s41586-020-2294-9

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Article

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform.

Nature - 1 Jun 2020

Thi Nhu Thao Tran, Labroussaa Fabien, Ebert Nadine, V'kovski Philip, Stalder Hanspeter, Portmann Jasmine, Kelly Jenna, Steiner Silvio, Holwerda Melle, Kratzel Annika, Gultom Mitra, Schmied Kimberly, Laloli Laura, Hüsser Linda, Wider Manon, Pfaender Stephanie, Hirt Dagny, Cippà Valentina, Crespo-Pomar Silvia, Schröder Simon, Muth Doreen, Niemeyer Daniela, Corman Victor M, Müller Marcel A, Drosten Christian, Dijkman Ronald, Jores Joerg, Thiel Volker

Abstract excerpt

Reverse genetics has been an indispensable tool to gain insights into viral pathogenesis and vaccine development. The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate in Escherichia coli owing to the size and occasional instability of the genome1-3. Therefore, an alternative rapid and robust reverse-genetics platform for RNA viruses would benefit the research...

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PMID: 32365353
DOI: 10.1038/s41586-020-2294-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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