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Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols

29 Oct 2021

Abstract excerpt

Surveillance of the SARS-CoV-2 variants including the quickly spreading mutants by rapid and near real-time sequencing of the viral genome provides an important tool for effective health policy decision making in the ongoing COVID-19 pandemic. Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for analysis of the SARS-CoV-2...

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Share to Advanced biosensing and bioanalysis techniques
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PMID: 34714886
DOI: 10.1371/journal.pone.0259277

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