Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation.

Abstract excerpt

We present nanopore adapter-enriched Cas9-targeted sequencing (nAECATS), a method permitting inexpensive, ultra-deep, selective long-read sequencing of targeted regions in native, unamplified DNA. This method modifies previous Cas9-targeted sequencing approaches through the inclusion of a bead-based capture step that exploits the poly(T)8 stretch within the R10.4.1 ligation adapter for additional purification....

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