Article
Ultralow-input single-tube linked-read library method enables short-read second-generation sequencing systems to routinely generate highly accurate and economical long-range sequencing information.
Genome research - 1 Jun 2020
Chen Zhoutao, Pham Long, Wu Tsai-Chin, Mo Guoya, Xia Yu, Chang Peter L, Porter Devin, Phan Tan, Che Huu, Tran Hao, Bansal Vikas, Shaffer Justin, Belda-Ferre Pedro, Humphrey Greg, Knight Rob, Pevzner Pavel, Pham Son, Wang Yong, Lei Ming
Abstract excerpt
Long-range sequencing information is required for haplotype phasing, de novo assembly, and structural variation detection. Current long-read sequencing technologies can provide valuable long-range information but at a high cost with low accuracy and high DNA input requirements. We have developed a single-tube Transposase Enzyme Linked Long-read Sequencing (TELL-seq) technology, which enables a low-cost,...
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