Concanavalin A-assisted extraction-free one-pot RPA-CRISPR/Cas12a assay for rapid detection of HPV16

Abstract excerpt

<title>Abstract</title> <p>Human papillomavirus (HPV) infection is a major threat to women’s health worldwide. High-risk subtypes, particularly HPV16, require rigorous screening and long-term surveillance to control cervical cancer. However, traditional HPV testing is hampered by the need for nucleic acid extraction, reliance on specialized technicians, and fluorescence detection equipment, limiting its suitabili...

Topics

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• Biosensing Techniques
• CRISPR-Cas Systems
• DNA, Viral
• Genotype
• Nucleic Acid Amplification Techniques
• Papillomaviridae
• Papillomavirus Infections
• Recombinases

Identifiers and source

Literature Corpus work

c1392ecf-22a1-5b7c-97b7-a38e6ad43e73

DOI

10.21203/rs.3.rs-6278232/v1