Article
A Cas12a-based fluorescent microfluidic system for rapid on-site human papillomavirus diagnostics.
Applied microbiology and biotechnology - 1 Oct 2023
Huang Baicheng, Lou Yufeng, Zeng Zihan, Kan Xingchi, Shi Xinping, Wu Yue, Guo Ling, Wang Muzhen, Huang Xingxu, Tian Xuemei, Wang Xinjie
Abstract excerpt
Persistent infection with human papillomavirus (HPV) is the leading cause of cervical cancer, and early diagnosis is crucial for clinical management. However, the easy and rapid on-site diagnostic for HPV genotyping remains challenging. Here, we develop a Cas12a-based fluorescent microfluidic detection system for diagnosing six HPV subtypes (HPV6, HPV11, HPV16, HPV18, HPV31, and HPV33). A panel of crRNAs and...
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