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Article

GoT-Splice protocol for multi-omics profiling of gene expression, cell-surface proteins, mutational status, and RNA splicing in human cells.

STAR protocols - 21 Jun 2024

Ganesan Saravanan, Cortés-López Mariela, Swett Ariel D, Dai Xiaoguang, Hickey Scott, Chamely Paulina, Hawkins Allegra G, Juul Sissel, Landau Dan A, Gaiti Federico

Abstract excerpt

Studying RNA splicing factor mutations is challenging due to difficulties in distinguishing wild-type and mutant cells within complex human tissues and inaccuracies associated with reconstructing splicing signals from short-read sequencing data. Here, we present Genotyping of Transcriptomes (GoT)-Splice, a protocol that overcomes these limitations by combining GoT with enhanced long-read single-cell transcriptome...

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Identifiers

PMID: 38512867
DOI: 10.1016/j.xpro.2024.102966
PMCID: PMC10966806

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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