Article

Human insulin gene expressing with Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) expression system

2011-02-01

Abstract excerpt

Using human genomic DNA as a template, the human insulin gene was cloned and used to construct various reBmMNPVbacmids. Cysteine protease gene deletion (CPD-BmMNPV bacmid) and cysteine protease- and chitinase-deficient (CPPD- BmMNPV bacmid) baculoviruses were used to express both native and FLAG-tagged human insulin. Silkworm larvae were infected with the above recombinant bacmid DNAs, and the expressed insulin wa...

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Literature Corpus work: bab6dace-81ff-59cc-aa75-ba5a8f70f058
DOI: 10.1007/s11274-010-0470-z

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