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Transient Expression and Subcellular Targeting of Human Insulin Using a Plant Virus-Based Expression System In Planta

2025-10-20

Abstract excerpt

A plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus (Potyvirus cucurbitaflavitesselati; ZYMV) was used to express the human insulin in planta. The proinsulin gene was in frame inserted between the P1 and HC-Pro genes of the ZYMV vector. Six histidine residues and a NIa protease cleavage site were added at the C-terminal region of the inserts to facilitate detection and...

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Identifiers and source

Literature Corpus work
5985ddff-5fc0-5bbb-b2e8-fe5566d2d099
DOI
10.20944/preprints202510.1570.v1
Open publication

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Transient Expression and Subcellular Targeting of Human Insulin Using a Plant Virus-Based Expression System In PlantaDOI 10.20944/preprints202510.1570.v1
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