Article

Efficient detection of pre-proinsulin by double antibody sandwich ELISA

2020-03-29

Abstract excerpt

<h4>Abstract Objective</h4> To generate monoclonal antibodies against pre-proinsulin (PPI), and establish sandwich ELISA method to provide a basis for PPI detection in recombinant human insulin production. <h4>Methods</h4> The Balb /c mice were immunized with PPI, and the hybridomas secreting anti-PPI monoclonal antibodies were obtained by conventional cell fusion technique and ELISA screening.The antibody was p...

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Literature Corpus work: 5a601f4c-6a9d-5e93-8ffc-62506e436dd5
DOI: 10.1101/2020.03.27.011098

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