Article
Expression and identification of mutated osteoprotegerin in culture cells and larvae of silkworm.
Acta biochimica et biophysica Sinica - 1 May 2004
Xiao Hai-Long, Jin Yong-Feng, Zhang Yao-Zhou
Abstract excerpt
The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the recombinant plasmid was co-transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous recombination occurred inside the cells. The recombinant virus BmNPV-OPG-372 was screened and identified by Southern blotting. The recombinant human OPG-372 was expressed in cultured cells and the...
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