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Article

Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging

8 Jul 2022

Abstract excerpt

The African turquoise killifish Nothobranchius furzeri (N. furzeri) is a useful model organism for studying aging, age-related diseases, and embryonic diapause. CRISPR/Cas9-mediated gene knockout and Tol2 transposon-mediated transgenesis in N. furzeri have been reported previously. However, these methods take time to generate knockout and transgenic fish. In addition, knock-in technology that inserts large DNA...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 35804091
DOI: 10.1038/s41598-022-15972-3

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