Article

Physiologically refined cell culture conditions uncover oncogene-dependent metabolic signatures in Ewing sarcoma spheroids

2024-07-09

Abstract excerpt

<h4>SUMMARY</h4> Ewing sarcoma (EwS) cell line culture largely relies on standard techniques, which do not recapitulate physiological conditions. Here, we report on a physiologically improved, feasible, and cost-efficient EwS cell culture technique employing an advanced medium composition, reduced fetal calf serum, and spheroidal growth. Functional in vitro assays and transcriptome profiling demonstrated that th...

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Literature Corpus work: b7bf73fb-826c-5369-8685-56ccf5582e57
DOI: 10.1101/2024.07.05.602211

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