Article

Protocol for Seahorse 3D Mito Stress assay in patient-derived pediatric brain tumor single neurospheres

2025-12-05

Abstract excerpt

<h4>Summary</h4> Metabolism is essential for cellular functions and is often altered in cancer. While Seahorse assays are well established for measuring metabolic changes in 2D cell cultures, their application to 3D models remains challenging. Here, we present a step-by-step protocol for plating individual brain tumor neurospheres in the assay microplate to measure their bioenergetics. We provide actionable recom...

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Literature Corpus work: cbef8efd-ff6e-5483-bf65-4bf6b7ed255b
DOI: 10.64898/2025.12.03.691986

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