Article

Correlative SHG-AFM imaging workflow for label-free quantitative analysis of collagen structure–function relationships

2026-06-07

Abstract excerpt

We present a user-friendly correlative second harmonic generation (SHG) and atomic force microscopy (AFM) imaging workflow for quantifying the nanomechanical properties of collagen in unfixed, unlabeled tissue sections. SHG Aligned Profiling for Elasticity and Segmentation or SHAPES utilizes SHG imaging to guide AFM force mapping, enabling label-free, anatomically specific selection of regions of interest, facilit...

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Literature Corpus work: b5b75b04-d4a1-5687-819c-af0c81f9f6c7
DOI: 10.64898/2026.06.04.727022

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