Article

Reversing transgene silencing via targeted chromatin editing

2025-10-30

Abstract excerpt

Mammalian cell engineering offers the opportunity to uncover biological principles and develop next-generation biotechnologies. However, epigenetic silencing of transgenes hinders the control of gene expression in mammalian cells. Here, we use chromatin editing of an integrated reporter in CHO-K1 and human induced pluripotent stem cells to study the molecular interactions driving silencing and its reversal. After...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b38e6f18-f90c-577d-920c-9ad5d577e2b9
DOI: 10.1101/2025.10.28.685244

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Reversing transgene silencing via targeted chromatin editingDOI 10.1101/2025.10.28.685244Read

Concise Review: Human Cell Engineering: Cellular Reprogramming and Genome EditingSemantic proximity Read

Prevention of Transgene Silencing During Human Pluripotent Stem Cell DifferentiationSemantic proximity Read

Epigenetic reprogramming and DNA demethylationSemantic proximity Read

Direct readout of neural stem cell transgenesis with an integration-coupled gene expression switchSemantic proximity Read

How to train your cell - Towards controlling phenotypes by harnessing the epigenome of Chinese hamster ovary production cell lines.Semantic proximity Read

Phenotype switching through epigenetic conversion.Semantic proximity Read

Aberrant epigenetic silencing is triggered by a transient reduction in gene expression.Semantic proximity Read

Reversing DNA methylation by heterokaryon-mediated reprogrammingSemantic proximity Read

Nuclear reprogramming in mammalian somatic cell nuclear cloningSemantic proximity Read

Reprogramming mediated by cell fusion technology.Semantic proximity Read

Embryonic Development following Somatic Cell Nuclear Transfer Impeded by Persisting Histone MethylationSemantic proximity Read

Modulating the epigenetic state promotes the reprogramming of transformed cells to pluripotency in a line-specific mannerSemantic proximity Read