Article

Recyclable CRISPR/Cas9 mediated gene disruption and deletions in <i>Histoplasma</i>

2023-07-05

Abstract excerpt

Targeted gene disruption is challenging in the dimorphic fungal pathogen Histoplasma due to the low frequency of homologous recombination. Transformed DNA is either integrated ectopically into the genome or maintained extra chromosomally by de novo addition of telomeric sequences. Based on a system developed in Blastomyces , we adapted a CRISPR/Cas9 system to facilitate targeted gene disruption in Histoplasma...

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Literature Corpus work: b0916174-52dc-57d6-9778-f0ec575dca2b
DOI: 10.1101/2023.07.05.547774

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