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Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus Rhizopus microsporus

1 Dec 2021

Abstract excerpt

Mucormycosis is a lethal and emerging disease that has lacked a genetic model fulfilling both high virulence and the possibility of performing stable and reproducible gene manipulation by homologous recombination (HR). Here, we developed a new methodology to successfully perform HR in Rhizopus microsporus. We isolated an uracil auxotrophic recipient strain and optimized the critical steps in the genetic...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 35475217
DOI: 10.1016/j.crmeth.2021.100124

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