Article

Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy

2025-03-03

Abstract excerpt

Recombinant proteins are critical for modern therapeutics and diagnostics, with Chinese hamster ovary (CHO) cells serving as the primary production platform. However, environmental and chemical stressors in bioreactors often trigger cell death, particularly apoptosis, posing a significant challenge to recombinant protein manufacturing. Rapid, label-free methods to monitor cell death are essential for ensuring bett...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: acc1b724-0393-5d67-af6d-e3b0b2fca6bd
DOI: 10.1101/2025.03.01.641010

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering MicroscopyDOI 10.1101/2025.03.01.641010Read

Dissecting cell death pathways in fed‐batch bioreactorsSemantic proximity Read

Imaging vs Nonimaging Raman Spectroscopy for High-Throughput Single-Cell Phenotyping.Semantic proximity Read

Unbounded Cell Growth and Proteome Imbalance in Doxorubicin-Induced Senescent RPE-1 CellsSemantic proximity Read

Phenotype Identification of HeLa Cells Knockout CDK6 Gene Based on Label-Free Raman Imaging.Semantic proximity Read

Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis.Semantic proximity Read

Rapid residual bead quantification for cell therapy manufacturing using Raman spectroscopySemantic proximity Read

Label-free classification of cell death pathways via holotomography-based deep learning frameworkSemantic proximity Read

Cell Painting phenomics reveals size-dependent phenotypic responses to titanium dioxide nanoparticles in HepG2 cells.Semantic proximity Read

Stimulated Emission Depletion Imaging Reveals Mitochondrial Phenotypic Heterogeneity under Apoptosis Stimuli across Living Glioma Models.Semantic proximity Read

SLAPSHOT reveals rapid dynamics of extracellularly exposed proteome in response to calcium-activated plasma membrane phospholipid scramblingSemantic proximity Read

Engineering death resistance in CHO cells for improved perfusion cultureSemantic proximity Read

Development of high content imaging methods for cell death detection in human pluripotent stem cell-derived cardiomyocytes.Semantic proximity Read