Article
Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages.
mBio - 26 May 2015
Monk Ian R, Tree Jai J, Howden Benjamin P, Stinear Timothy P, Foster Timothy J
Abstract excerpt
UNLABELLED: Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal...
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