Article

Longstanding Transcriptional Activation of APOA1 and PON1 in Human Hepatocytes by CRISPR/dCas9 Technology—Transcriptomic Profile and Crosstalk with Endothelial Cells

2026-06-10

Abstract excerpt

Apolipoprotein A1 (APOA1) and paraoxonase 1 (PON1) are key proteins of high-density lipoprotein (HDL). The aim of the present study was to obtain and characterize an in vitro model for endogenous APOA1 and PON1 long-time up-regulation in hepatocytes that can be further used to decipher the mechanism of their protective action. Cultured human hepatocytes (HuH-7 cell line) were transfected with CRISPR/dCas9 activati...

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Literature Corpus work: abaceb96-727e-5fc6-b03f-9bdd2e282152
DOI: 10.20944/preprints202606.0792.v1

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