Article

Development of a versatile LCM-Seq method for spatial transcriptomics of fluorescently-tagged cholinergic neuron populations

2023-03-03

Abstract excerpt

Single-cell transcriptomics are powerful tools to define neuronal cell types based on co-expressed gene clusters. Limited RNA input in these technologies necessarily compromises transcriptome coverage and accuracy of differential expression analysis. We propose that bulk RNA-sequencing of neuronal pools defined by spatial position offers an alternative strategy to overcome these technical limitations. We report an...

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Literature Corpus work: ab004b67-a7ab-5c8e-adfa-3ae6b009234d
DOI: 10.1101/2023.03.02.530916

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