Article

State-dependent mapping of GlyR-cholesterol interactions by coupling crosslinking with mass spectrometry

2020-07-06

Abstract excerpt

Pentameric ligand-gated ion channel (pLGIC) allostery is dependent on dynamic associations with its diverse environment. The cellular membrane’s lipid composition influences channel function with cholesterol being a key regulator of channel activity. Human α 1 glycine receptor (GlyR) was purified from baculovirus infected insect cells and reconstituted in unilamellar vesicles at physiological cholesterol:lipid ra...

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Literature Corpus work: aad96864-c522-540f-9c83-518fcb8299b3
DOI: 10.1101/2020.07.02.185280

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