Article

Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq

2022-07-17

Abstract excerpt

Genomic and proteomic screens have identified numerous host factors of SARS-CoV-2, but efficient delineation of their molecular roles during infection remains a challenge. Here we use Perturb-seq, combining genetic perturbations with a single-cell readout, to investigate how inactivation of host factors changes the course of SARS-CoV-2 infection and the host response in human lung epithelial cells. Our high-dimens...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a527298e-4171-5575-a223-7da005d569c3
DOI: 10.1101/2022.07.15.500120

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seqDOI 10.1101/2022.07.15.500120Read

Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seqSemantic proximity Read

Global siRNA Screen Reveals Critical Human Host Factors of SARS-CoV-2 Multicycle ReplicationSemantic proximity Read

Global siRNA screen identifies human host factors critical for SARS-CoV-2 replication and late stages of infectionSemantic proximity Read

Identification and Targeting of Regulators of SARS-CoV-2-Host Interactions in the Airway EpitheliumSemantic proximity Read

Defining the host dependencies and the transcriptional landscape of RSV infection and bystander activationSemantic proximity Read

Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infectionSemantic proximity Read

Systematic Genome-Scale Identification of Host Factors for SARS-CoV-2 Infection Across Models Yields a Core Single Gene Dependency; <i>Ace2</i>Semantic proximity Read

Profiling transcription factor sub-networks in type I interferon signaling and in response to SARS-CoV-2 infectionSemantic proximity Read

Functional Landscape of SARS-CoV-2 Cellular RestrictionSemantic proximity Read

SARS-CoV-2 Nsp2 reprograms host immunity to drive pathogenic inflammationSemantic proximity Read

Interactome profiling reveals interaction of SARS-CoV-2 NSP13 with host factor STAT1 to suppress interferon signalingSemantic proximity Read

Resolving <i>in vitro</i> heterogeneity of host functional responses to HCoV-229E via single-cell analysesSemantic proximity Read