Article

Identifying phenotype-genotype-function coupling in 3D organoid imaging using Shape, Appearance and Motion Phenotype Observation Tool (SPOT)

2025-11-29

Abstract excerpt

Live cells in tissue are plastic, phenotypically dynamic, and modify their function in response to genetic and environmental perturbations. To unleash the power of live-cell imaging to identify phenotype-genotype-function coupling over time, we report the development of a standardized Shape-Appearance-Motion (SAM) “phenome” and SAM-Phenotype-Observation-Tool (SPOT), that act as an image-“transcriptome” and image-“...

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Literature Corpus work: a1f61f5c-be26-5b4b-ae85-3e0b6dc3ff67
DOI: 10.1101/2025.11.28.691111

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