Article

Helical remodeling augments 5-lipoxygenase activity

2022-03-31

Abstract excerpt

<h4>ABSTRACT</h4> The synthesis of pro-inflammatory leukotrienes implicated in asthma, allergic rhinitis, and atherosclerosis is initiated by the enzyme 5-lipoxygenase (5-LOX). The crystal structure of human Stable-5-LOX revealed a conformation where the catalytic iron was inaccessible to bulk solvent as two aromatic residues on a conserved helix-α2 (Hα2) plugged the substrate access portal. Here, we present a ne...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a193a928-1c6b-58ed-b57e-9f6c0b4ce1a4
DOI: 10.1101/2022.03.31.486615

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Helical remodeling augments 5-lipoxygenase activityDOI 10.1101/2022.03.31.486615Read

Human 15-LOX-1 active site mutations alter inhibitor binding and decrease potency.Semantic proximity Read

Molecular dynamics simulations to decipher the hotspots at the allosteric site of human 5-lipoxygenase.Semantic proximity Read

Conversion of pro-inflammatory murine Alox5 into an anti-inflammatory 15S-lipoxygenating enzyme by multiple mutations of sequence determinants.Semantic proximity Read

Ligand-induced formation of transient dimers of mammalian 12/15-lipoxygenase: a key to allosteric behavior of this class of enzymes?Semantic proximity Read

The biology of 5-lipoxygenase: function, structure, and regulatory mechanisms.Semantic proximity Read

Mutagenesis, Hydrogen-Deuterium Exchange, and Molecular Docking Investigations Establish the Dimeric Interface of Human Platelet-Type 12-Lipoxygenase.Semantic proximity Read

Crystal structure of a lipoxygenase in complex with substrate: the arachidonic acid-binding site of 8R-lipoxygenase.Semantic proximity Read

Role of Arg403 for thermostability and catalytic activity of rabbit 12/15-lipoxygenase.Semantic proximity Read

Probing dimerization and structural flexibility of mammalian lipoxygenases by small-angle X-ray scattering.Semantic proximity Read

Allosteric Ensembles Elucidate Mechanisms of Inhibition in Human Soluble Epoxide HydrolaseSemantic proximity Read

Alterations of lipoxygenase specificity by targeted substrate modification and site-directed mutagenesis.Semantic proximity Read

Mutations of Triad Determinants Changes the Substrate Alignment at the Catalytic Center of Human ALOX5.Semantic proximity Read