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Mutagenesis, Hydrogen-Deuterium Exchange, and Molecular Docking Investigations Establish the Dimeric Interface of Human Platelet-Type 12-Lipoxygenase.

Biochemistry - 16 Mar 2021

Tsai Wan-Chen, Aleem Ansari Mukhtar, Whittington Chris, Cortopassi Wilian A, Kalyanaraman Chakrapani, Baroz Angel, Iavarone Anthony T, Skrzypczak-Jankun Ewa, Jacobson Matthew P, Offenbacher Adam R, Holman Theodore

Abstract excerpt

It was previously shown that human platelet 12S-lipoxygenase (h12-LOX) exists as a dimer; however, the specific structure is unknown. In this study, we create a model of the dimer through a combination of computational methods, experimental mutagenesis, and hydrogen-deuterium exchange (HDX) investigations. Initially, Leu183 and Leu187 were replaced by negatively charged glutamate residues and neighboring aromatic...

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PMID: 33635645
DOI: 10.1021/acs.biochem.1c00053

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